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  • Imagem protegida – Proibida a reprodução
  • Imagem protegida – Proibida a reprodução
  • Imagem protegida – Proibida a reprodução

Reflection imaging of China ink-perfused brain vasculature using confocal laser-scanning microscopy after clarification of brain tissue by the Spalteholz method

Não Publicado

Robson Campos Gutierre, Diego Vannucci Campos, Renato Aarruda Mortara, Antônio Augusto Coppi, Ricardo Mario Arida

J Anat. 2017 Jan 5.[Epub ahead of print]

 

http://onlinelibrary.wiley.com/doi/10.1111/joa.12578/abstract;jsessionid=69981E984C48F8E7D4A86454091F743A.f03t03

Abstract

Confocal laser-scanning microscopy is a useful tool for visualizing neurons and glia in transparent preparations of brain tissue from laboratory animals. Currently, imaging capillaries and venules in transparent brain tissues requires the use of fluorescent proteins. Here, we show that vessels can be imaged by confocal laser-scanning microscopy in transparent cortical, hippocampal and cerebellar preparations after clarification of China ink-injected specimens by the Spalteholz method. This method may be suitable for global, three-dimensional, quantitative analyses of vessels, including stereological estimations of total volume and length and of surface area of vessels, which constitute indirect approaches to investigate angiogenesis.

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